Whole-genome bisulfite DNA sequencing of a DNMT3B mutant patient.

TitleWhole-genome bisulfite DNA sequencing of a DNMT3B mutant patient.
Publication TypeJournal Article
Year of Publication2012
AuthorsHeyn, H, Vidal, E, Sayols, S, Sanchez-Mut, JV, Moran, S, Medina, I, Sandoval, J, Simó-Riudalbas, L, Szczesna, K, Huertas, D, Gatto, S, Matarazzo, MR, Dopazo, J, Esteller, M
Date Published2012 Jun 01
KeywordsB-Lymphocytes; Cell Line, Transformed; Child, Preschool; DNA (Cytosine-5-)-Methyltransferases; DNA Methylation; Epigenesis, Genetic; Face; Female; Genome, Human; High-Throughput Nucleotide Sequencing; Humans; Immunologic Deficiency Syndromes; mutation; Primary Immunodeficiency Diseases; Sequence Analysis, DNA; Sulfites

The immunodeficiency, centromere instability and facial anomalies (ICF) syndrome is associated to mutations of the DNA methyl-transferase DNMT3B, resulting in a reduction of enzyme activity. Aberrant expression of immune system genes and hypomethylation of pericentromeric regions accompanied by chromosomal instability were determined as alterations driving the disease phenotype. However, so far only technologies capable to analyze single loci were applied to determine epigenetic alterations in ICF patients. In the current study, we performed whole-genome bisulphite sequencing to assess alteration in DNA methylation at base pair resolution. Genome-wide we detected a decrease of methylation level of 42%, with the most profound changes occurring in inactive heterochromatic regions, satellite repeats and transposons. Interestingly, transcriptional active loci and ribosomal RNA repeats escaped global hypomethylation. Despite a genome-wide loss of DNA methylation the epigenetic landscape and crucial regulatory structures were conserved. Remarkably, we revealed a mislocated activity of mutant DNMT3B to H3K4me1 loci resulting in hypermethylation of active promoters. Functionally, we could associate alterations in promoter methylation with the ICF syndrome immunodeficient phenotype by detecting changes in genes related to the B-cell receptor mediated maturation pathway.

Alternate JournalEpigenetics
PubMed ID22595875
PubMed Central IDPMC3398983
Grant List268626 / ER / European Research Council / International