Highly sensitive and ultrafast read mapping for RNA-seq analysis.

TitleHighly sensitive and ultrafast read mapping for RNA-seq analysis.
Publication TypeJournal Article
Year of Publication2016
AuthorsMedina, I, Tárraga, J, Martínez, H, Barrachina, S, Castillo, MI, Paschall, J, Salavert-Torres, J, Blanquer-Espert, I, Hernández-García, V, Quintana-Ortí, ES, Dopazo, J
JournalDNA Res
Date Published2016 Apr
KeywordsGenomics; High-Throughput Nucleotide Sequencing; Humans; Sensitivity and Specificity; Sequence Analysis, RNA; Transcriptome

As sequencing technologies progress, the amount of data produced grows exponentially, shifting the bottleneck of discovery towards the data analysis phase. In particular, currently available mapping solutions for RNA-seq leave room for improvement in terms of sensitivity and performance, hindering an efficient analysis of transcriptomes by massive sequencing. Here, we present an innovative approach that combines re-engineering, optimization and parallelization. This solution results in a significant increase of mapping sensitivity over a wide range of read lengths and substantial shorter runtimes when compared with current RNA-seq mapping methods available.

Alternate JournalDNA Res
PubMed ID26740642
PubMed Central IDPMC4833417