Expression and microarrays.

TitleExpression and microarrays.
Publication TypeJournal Article
Year of Publication2008
AuthorsDopazo, J, Al-Shahrour, F
JournalMethods Mol Biol
Date Published2008
KeywordsAnimals; Computational Biology; gene expression; Gene Expression Profiling; Humans; Oligonucleotide Array Sequence Analysis

High throughput methodologies have increased by several orders of magnitude the amount of experimental microarray data available. Nevertheless, translating these data into useful biological knowledge remains a challenge. There is a risk of perceiving these methodologies as mere factories that produce never-ending quantities of data if a proper biological interpretation is not provided. Methods of interpreting these data are continuously evolving. Typically, a simple two-step approach has been used, in which genes of interest are first selected based on thresholds for the experimental values, and then enrichment in biologically relevant terms in the annotations of these genes is analyzed in a second step. For various reasons, such methods are quite poor in terms of performance and new procedures inspired by systems biology that directly address sets of functionally related genes are currently under development.

Alternate JournalMethods Mol Biol
PubMed ID18712307