Annotated draft genomic sequence from a Streptococcus pneumoniae type 19F clinical isolate

TitleAnnotated draft genomic sequence from a Streptococcus pneumoniae type 19F clinical isolate
Publication TypeJournal Article
Year of Publication2001
AuthorsDopazo, J, Mendoza, A, Herrero, J, Caldara, F, Humbert, Y, Friedli, L, Guerrier, M, Grand-Schenk, E, Gandin, C, de Francesco, M, Polissi, A, Buell, G, Feger, G, Garcia, E, Peitsch, M, Garcia-Bustos, JF
JournalMicrob Drug Resist
KeywordsBacterial Molecular Sequence Data Pneumococcal Infections/*microbiology Prokaryotic Cells RNA; Bacterial/chemistry/genetics Genes; Bacterial/genetics *Genome; DNA; Transfer/metabolism Streptococcus pneumoniae/*genetics

The public availability of numerous microbial genomes is enabling the analysis of bacterial biology in great detail and with an unprecedented, organism-wide and taxon-wide, broad scope. Streptococcus pneumoniae is one of the most important bacterial pathogens throughout the world. We present here sequences and functional annotations for 2.1-Mbp of pneumococcal DNA, covering more than 90% of the total estimated size of the genome. The sequenced strain is a clinical isolate resistant to macrolides and tetracycline. It carries a type 19F capsular locus, but multilocus sequence typing for several conserved genetic loci suggests that the strain sequenced belongs to a pneumococcal lineage that most often expresses a serotype 15 capsular polysaccharide. A total of 2,046 putative open reading frames (ORFs) longer than 100 amino acids were identified (average of 1,009 bp per ORF), including all described two-component systems and aminoacyl tRNA synthetases. Comparisons to other complete, or nearly complete, bacterial genomes were made and are presented in a graphical form for all the predicted proteins.


Dopazo, J Mendoza, A Herrero, J Caldara, F Humbert, Y Friedli, L Guerrier, M Grand-Schenk, E Gandin, C de Francesco, M Polissi, A Buell, G Feger, G Garcia, E Peitsch, M Garcia-Bustos, J F United States Microbial drug resistance (Larchmont, N.Y.) Microb Drug Resist. 2001 Summer;7(2):99-125.