<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Bogliolo, Massimo</style></author><author><style face="normal" font="default" size="100%">Pujol, Roser</style></author><author><style face="normal" font="default" size="100%">Aza-Carmona, Miriam</style></author><author><style face="normal" font="default" size="100%">Muñoz-Subirana, Núria</style></author><author><style face="normal" font="default" size="100%">Rodriguez-Santiago, Benjamin</style></author><author><style face="normal" font="default" size="100%">Casado, José Antonio</style></author><author><style face="normal" font="default" size="100%">Rio, Paula</style></author><author><style face="normal" font="default" size="100%">Bauser, Christopher</style></author><author><style face="normal" font="default" size="100%">Reina-Castillón, Judith</style></author><author><style face="normal" font="default" size="100%">Lopez-Sanchez, Marcos</style></author><author><style face="normal" font="default" size="100%">Gonzalez-Quereda, Lidia</style></author><author><style face="normal" font="default" size="100%">Gallano, Pia</style></author><author><style face="normal" font="default" size="100%">Catalá, Albert</style></author><author><style face="normal" font="default" size="100%">Ruiz-Llobet, Ana</style></author><author><style face="normal" font="default" size="100%">Badell, Isabel</style></author><author><style face="normal" font="default" size="100%">Diaz-Heredia, Cristina</style></author><author><style face="normal" font="default" size="100%">Hladun, Raquel</style></author><author><style face="normal" font="default" size="100%">Senent, Leonort</style></author><author><style face="normal" font="default" size="100%">Argiles, Bienvenida</style></author><author><style face="normal" font="default" size="100%">Bergua Burgues, Juan Miguel</style></author><author><style face="normal" font="default" size="100%">Bañez, Fatima</style></author><author><style face="normal" font="default" size="100%">Arrizabalaga, Beatriz</style></author><author><style face="normal" font="default" size="100%">López Almaraz, Ricardo</style></author><author><style face="normal" font="default" size="100%">Lopez, Monica</style></author><author><style face="normal" font="default" size="100%">Figuera, Ángela</style></author><author><style face="normal" font="default" size="100%">Molinés, Antonio</style></author><author><style face="normal" font="default" size="100%">Pérez de Soto, Inmaculada</style></author><author><style face="normal" font="default" size="100%">Hernando, Inés</style></author><author><style face="normal" font="default" size="100%">Muñoz, Juan Antonio</style></author><author><style face="normal" font="default" size="100%">Del Rosario Marin, Maria</style></author><author><style face="normal" font="default" size="100%">Balmaña, Judith</style></author><author><style face="normal" font="default" size="100%">Stjepanovic, Neda</style></author><author><style face="normal" font="default" size="100%">Carrasco, Estela</style></author><author><style face="normal" font="default" size="100%">Cuesta, Isabel</style></author><author><style face="normal" font="default" size="100%">Cosuelo, José Miguel</style></author><author><style face="normal" font="default" size="100%">Regueiro, Alexandra</style></author><author><style face="normal" font="default" size="100%">Moraleda Jimenez, José</style></author><author><style face="normal" font="default" size="100%">Galera-Miñarro, Ana Maria</style></author><author><style face="normal" font="default" size="100%">Rosiñol, Laura</style></author><author><style face="normal" font="default" size="100%">Carrió, Anna</style></author><author><style face="normal" font="default" size="100%">Beléndez-Bieler, Cristina</style></author><author><style face="normal" font="default" size="100%">Escudero Soto, Antonio</style></author><author><style face="normal" font="default" size="100%">Cela, Elena</style></author><author><style face="normal" font="default" size="100%">de la Mata, Gregorio</style></author><author><style face="normal" font="default" size="100%">Fernández-Delgado, Rafael</style></author><author><style face="normal" font="default" size="100%">Garcia-Pardos, Maria Carmen</style></author><author><style face="normal" font="default" size="100%">Sáez-Villaverde, Raquel</style></author><author><style face="normal" font="default" size="100%">Barragaño, Marta</style></author><author><style face="normal" font="default" size="100%">Portugal, Raquel</style></author><author><style face="normal" font="default" size="100%">Lendinez, Francisco</style></author><author><style face="normal" font="default" size="100%">Hernadez, Ines</style></author><author><style face="normal" font="default" size="100%">Vagace, José Manue</style></author><author><style face="normal" font="default" size="100%">Tapia, Maria</style></author><author><style face="normal" font="default" size="100%">Nieto, José</style></author><author><style face="normal" font="default" size="100%">Garcia, Marta</style></author><author><style face="normal" font="default" size="100%">Gonzalez, Macarena</style></author><author><style face="normal" font="default" size="100%">Vicho, Cristina</style></author><author><style face="normal" font="default" size="100%">Galvez, Eva</style></author><author><style face="normal" font="default" size="100%">Valiente, Alberto</style></author><author><style face="normal" font="default" size="100%">Antelo, Maria Luisa</style></author><author><style face="normal" font="default" size="100%">Ancliff, Phil</style></author><author><style face="normal" font="default" size="100%">García, Francisco</style></author><author><style face="normal" font="default" size="100%">Dopazo, Joaquin</style></author><author><style face="normal" font="default" size="100%">Sevilla, Julian</style></author><author><style face="normal" font="default" size="100%">Paprotka, Tobias</style></author><author><style face="normal" font="default" size="100%">Pérez-Jurado, Luis Alberto</style></author><author><style face="normal" font="default" size="100%">Bueren, Juan</style></author><author><style face="normal" font="default" size="100%">Surralles, Jordi</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Optimised molecular genetic diagnostics of Fanconi anaemia by whole exome sequencing and functional studies.</style></title><secondary-title><style face="normal" font="default" size="100%">J Med Genet</style></secondary-title><alt-title><style face="normal" font="default" size="100%">J Med Genet</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cell Line</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA Copy Number Variations</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA Repair</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA-Binding Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Fanconi Anemia</style></keyword><keyword><style  face="normal" font="default" size="100%">Fanconi Anemia Complementation Group A Protein</style></keyword><keyword><style  face="normal" font="default" size="100%">Female</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene Knockout Techniques</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic Predisposition to Disease</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Male</style></keyword><keyword><style  face="normal" font="default" size="100%">Mutation, Missense</style></keyword><keyword><style  face="normal" font="default" size="100%">Polymorphism, Single Nucleotide</style></keyword><keyword><style  face="normal" font="default" size="100%">whole exome sequencing</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2020 04</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">57</style></volume><pages><style face="normal" font="default" size="100%">258-268</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&lt;b&gt;PURPOSE: &lt;/b&gt;Patients with Fanconi anaemia (FA), a rare DNA repair genetic disease, exhibit chromosome fragility, bone marrow failure, malformations and cancer susceptibility. FA molecular diagnosis is challenging since FA is caused by point mutations and large deletions in 22 genes following three heritability patterns. To optimise FA patients' characterisation, we developed a simplified but effective methodology based on whole exome sequencing (WES) and functional studies.&lt;/p&gt;&lt;p&gt;&lt;b&gt;METHODS: &lt;/b&gt;68 patients with FA were analysed by commercial WES services. Copy number variations were evaluated by sequencing data analysis with RStudio. To test  missense variants, wt FANCA cDNA was cloned and variants were introduced by site-directed mutagenesis. Vectors were then tested for their ability to complement DNA repair defects of a FANCA-KO human cell line generated by TALEN technologies.&lt;/p&gt;&lt;p&gt;&lt;b&gt;RESULTS: &lt;/b&gt;We identified 93.3% of mutated alleles including large deletions. We determined the pathogenicity of three FANCA missense variants and demonstrated that two  variants reported in mutations databases as 'affecting functions' are SNPs. Deep analysis of sequencing data revealed patients' true mutations, highlighting the importance of functional analysis. In one patient, no pathogenic variant could be identified in any of the 22 known FA genes, and in seven patients, only one deleterious variant could be identified (three patients each with FANCA and FANCD2 and one patient with FANCE mutations) CONCLUSION: WES and proper bioinformatics analysis are sufficient to effectively characterise patients with FA regardless of the rarity of their complementation group, type of mutations, mosaic condition and DNA source.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><custom1><style face="normal" font="default" size="100%">https://www.ncbi.nlm.nih.gov/pubmed/31586946?dopt=Abstract</style></custom1></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Yung, Sun</style></author><author><style face="normal" font="default" size="100%">Ledran, Maria</style></author><author><style face="normal" font="default" size="100%">Moreno-Gimeno, Inmaculada</style></author><author><style face="normal" font="default" size="100%">Conesa, Ana</style></author><author><style face="normal" font="default" size="100%">Montaner, David</style></author><author><style face="normal" font="default" size="100%">Dopazo, Joaquin</style></author><author><style face="normal" font="default" size="100%">Dimmick, Ian</style></author><author><style face="normal" font="default" size="100%">Slater, Nicholas J</style></author><author><style face="normal" font="default" size="100%">Marenah, Lamin</style></author><author><style face="normal" font="default" size="100%">Real, Pedro J</style></author><author><style face="normal" font="default" size="100%">Paraskevopoulou, Iliana</style></author><author><style face="normal" font="default" size="100%">Bisbal, Viviana</style></author><author><style face="normal" font="default" size="100%">Burks, Deborah</style></author><author><style face="normal" font="default" size="100%">Santibanez-Koref, Mauro</style></author><author><style face="normal" font="default" size="100%">Moreno, Ruben</style></author><author><style face="normal" font="default" size="100%">Mountford, Joanne</style></author><author><style face="normal" font="default" size="100%">Menendez, Pablo</style></author><author><style face="normal" font="default" size="100%">Armstrong, Lyle</style></author><author><style face="normal" font="default" size="100%">Lako, Majlinda</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Large-scale transcriptional profiling and functional assays reveal important roles for Rho-GTPase signalling and SCL during haematopoietic differentiation of human embryonic stem cells.</style></title><secondary-title><style face="normal" font="default" size="100%">Hum Mol Genet</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Hum Mol Genet</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Acute Disease</style></keyword><keyword><style  face="normal" font="default" size="100%">Anemia, Hemolytic</style></keyword><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">Basic Helix-Loop-Helix Transcription Factors</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Differentiation</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Line</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Lineage</style></keyword><keyword><style  face="normal" font="default" size="100%">Cluster Analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Embryonic Stem Cells</style></keyword><keyword><style  face="normal" font="default" size="100%">Erythroid Cells</style></keyword><keyword><style  face="normal" font="default" size="100%">Flow Cytometry</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene Expression Profiling</style></keyword><keyword><style  face="normal" font="default" size="100%">Hematopoietic Stem Cells</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Mice</style></keyword><keyword><style  face="normal" font="default" size="100%">Myeloid Cells</style></keyword><keyword><style  face="normal" font="default" size="100%">Paracrine Communication</style></keyword><keyword><style  face="normal" font="default" size="100%">Proto-Oncogene Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Reverse Transcriptase Polymerase Chain Reaction</style></keyword><keyword><style  face="normal" font="default" size="100%">rho GTP-Binding Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Signal Transduction</style></keyword><keyword><style  face="normal" font="default" size="100%">Stem Cell Transplantation</style></keyword><keyword><style  face="normal" font="default" size="100%">T-Cell Acute Lymphocytic Leukemia Protein 1</style></keyword><keyword><style  face="normal" font="default" size="100%">Transcriptome</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2011 Dec 15</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">20</style></volume><pages><style face="normal" font="default" size="100%">4932-46</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Understanding the transcriptional cues that direct differentiation of human embryonic stem cells (hESCs) and human-induced pluripotent stem cells to defined and functional cell types is essential for future clinical applications. In this study, we have compared transcriptional profiles of haematopoietic progenitors derived from hESCs at various developmental stages of a feeder- and serum-free differentiation method and show that the largest transcriptional changes occur during the first 4 days of differentiation. Data mining on the basis of molecular function revealed Rho-GTPase signalling as a key regulator of differentiation. Inhibition of this pathway resulted in a significant reduction in the numbers of emerging haematopoietic progenitors throughout the differentiation window, thereby uncovering a previously unappreciated role for Rho-GTPase signalling during human haematopoietic development. Our analysis indicated that SCL was the 11th most upregulated transcript during the first 4 days of the hESC differentiation process. Overexpression of SCL in hESCs promoted differentiation to meso-endodermal lineages, the emergence of haematopoietic and erythro-megakaryocytic progenitors and accelerated erythroid differentiation. Importantly, intrasplenic transplantation of SCL-overexpressing hESC-derived haematopoietic cells enhanced recovery from induced acute anaemia without significant cell engraftment, suggesting a paracrine-mediated effect.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">24</style></issue><custom1><style face="normal" font="default" size="100%">https://www.ncbi.nlm.nih.gov/pubmed/21937587?dopt=Abstract</style></custom1></record></records></xml>