<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Gandia, M.</style></author><author><style face="normal" font="default" size="100%">A. Conesa</style></author><author><style face="normal" font="default" size="100%">Ancillo, G.</style></author><author><style face="normal" font="default" size="100%">J. Gadea</style></author><author><style face="normal" font="default" size="100%">J. Forment</style></author><author><style face="normal" font="default" size="100%">Pallas, V.</style></author><author><style face="normal" font="default" size="100%">Flores, R.</style></author><author><style face="normal" font="default" size="100%">Duran-Vila, N.</style></author><author><style face="normal" font="default" size="100%">Moreno, P.</style></author><author><style face="normal" font="default" size="100%">Guerri, J.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Transcriptional response of Citrus aurantifolia to infection by Citrus tristeza virus</style></title><secondary-title><style face="normal" font="default" size="100%">Virology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Citrus/*genetics/physiology/virology Closterovirus/genetics/*physiology Genes</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Oligonucleotide Array Sequence Analysis Reverse Transcriptase Polymerase Chain Reaction *Transcription</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2007</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;dopt=Citation&amp;list_uids=17617431</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">2</style></number><volume><style face="normal" font="default" size="100%">367</style></volume><pages><style face="normal" font="default" size="100%">298-306</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Changes in gene expression of Mexican lime plants in response to infection with a severe (T305) or a mild (T385) isolate of Citrus tristeza virus (CTV) were analyzed using a cDNA microarray containing 12,672 probes to 6875 different citrus genes. Statistically significant (P&lt;0.01) expression changes of 334 genes were detected in response to infection with isolate T305, whereas infection with T385 induced no significant change. Induced genes included 145 without significant similarity with known sequences and 189 that were classified in seven functional categories. Genes related with response to stress and defense were the main category and included 28% of the genes induced. Selected transcription changes detected by microarray analysis were confirmed by quantitative real-time RT-PCR. Changes detected in the transcriptome upon infecting lime with T305 may be associated either with symptom expression, with a strain-specific defense mechanism, or with a general response to stress.</style></abstract><notes><style face="normal" font="default" size="100%">Gandia, Monica Conesa, Ana Ancillo, Gema Gadea, Jose Forment, Javier Pallas, Vicente Flores, Ricardo Duran-Vila, Nuria Moreno, Pedro Guerri, Jose Research Support, Non-U.S. Gov’t United States Virology Virology. 2007 Oct 25;367(2):298-306. Epub 2007 Jul 9.</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Elena, S. F.</style></author><author><style face="normal" font="default" size="100%">Dopazo, J.</style></author><author><style face="normal" font="default" size="100%">de la Pena, M.</style></author><author><style face="normal" font="default" size="100%">Flores, R.</style></author><author><style face="normal" font="default" size="100%">Diener, T. O.</style></author><author><style face="normal" font="default" size="100%">Moya, A.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Phylogenetic analysis of viroid and viroid-like satellite RNAs from plants: a reassessment</style></title><secondary-title><style face="normal" font="default" size="100%">J Mol Evol</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Evolution</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular *Phylogeny Plant Viruses/*genetics RNA</style></keyword><keyword><style  face="normal" font="default" size="100%">Satellite/*genetics RNA</style></keyword><keyword><style  face="normal" font="default" size="100%">Viral/genetics Viroids/*genetics</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2001</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;dopt=Citation&amp;list_uids=11479686</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">2</style></number><volume><style face="normal" font="default" size="100%">53</style></volume><pages><style face="normal" font="default" size="100%">155-9</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The proposed monophyletic origin of a group of subviral plant pathogens (viroids and viroid-like satellite RNAs), as well as the phylogenetic relationships and the resulting taxonomy of these entities, has been recently questioned. The criticism comes from the (apparent) lack of sequence similarity among these RNAs necessary to reliably infer a phylogeny. Here we show that, despite their low overall sequence similarity, a sequence alignment manually adjusted to take into account all the local similarities and the insertions/deletions and duplications/rearrangements described in the literature for viroids and viroid-like satellite RNA, along with the use of an appropriate estimator of genetic distances, constitutes a data set suitable for a phylogenetic reconstruction. When the likelihood-mapping method was applied to this data set, the tree-likeness obtained was higher than that corresponding to a sequence alignment that does not take into consideration the local similarities. In addition, bootstrap analysis also supports the major groups previously proposed and the reconstruction is consistent with the biological properties of this RNAs.</style></abstract><notes><style face="normal" font="default" size="100%">Elena, S F Dopazo, J de la Pena, M Flores, R Diener, T O Moya, A Letter Research Support, Non-U.S. Gov’t United States Journal of molecular evolution J Mol Evol. 2001 Aug;53(2):155-9.</style></notes></record></records></xml>