TY - JOUR T1 - Filamentous fungi as cell factories for heterologous protein production JF - Trends Biotechnol Y1 - 2002 A1 - Punt, P. J. A1 - van Biezen, N. A1 - A. Conesa A1 - Albers, A. A1 - Mangnus, J. A1 - van den Hondel, C. KW - Fermentation/genetics/physiology Fungi/*genetics/*metabolism Humans Interleukin-6/analysis/*biosynthesis/genetics Peroxidases/analysis/*biosynthesis/genetics Protein Conformation Recombinant Proteins/analysis/*biosynthesis/genetics AB - Filamentous fungi have been used as sources of metabolites and enzymes for centuries. For about two decades, molecular genetic tools have enabled us to use these organisms to express extra copies of both endogenous and exogenous genes. This review of current practice reveals that molecular tools have enabled several new developments. But it has been process development that has driven the final breakthrough to achieving commercially relevant quantities of protein. Recent research into gene expression in filamentous fungi has explored their wealth of genetic diversity with a view to exploiting them as expression hosts and as a source of new genes. Inevitably, the progress in the ’genomics’ technology will further develop high-throughput technologies for these organisms. VL - 20 UR - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11943375 N1 - Punt, Peter J van Biezen, Nick Conesa, Ana Albers, Alwin Mangnus, Jeroen van den Hondel, Cees Review England Trends in biotechnology Trends Biotechnol. 2002 May;20(5):200-6. ER -