02327nas a2200241   4500008004100000245007500041210006900116260001300185300001100198490000700209520154000216100002401756700003301780700002101813700001901834700003501853700002001888700001801908700002701926700002001953700001701973856009501990       2011                        eng d00aRole of tomato BRANCHED1-like genes in the control of shoot branching.0 aRole of tomato BRANCHED1like genes in the control of shoot branc  c2011 Aug  a701-140 v673 a<p>In angiosperms, shoot branching greatly determines overall plant architecture and affects fundamental aspects of plant life. Branching patterns are determined by genetic pathways conserved widely across angiosperms. In Arabidopsis thaliana (Brassicaceae, Rosidae) BRANCHED1 (BRC1) plays a central role in this process, acting locally to arrest axillary bud growth. In tomato (Solanum lycopersicum, Solanaceae, Asteridae) we have identified two BRC1-like paralogues, SlBRC1a and SlBRC1b. These genes are expressed in arrested axillary buds and both are down-regulated upon bud activation, although SlBRC1a is transcribed at much lower levels than SlBRC1b. Alternative splicing of SlBRC1a renders two transcripts that encode two BRC1-like proteins with different C-t domains due to a 3’-terminal frameshift. The phenotype of loss-of-function lines suggests that SlBRC1b has retained the ancestral role of BRC1 in shoot branch suppression. We have isolated the BRC1a and BRC1b genes of other Solanum species and have studied their evolution rates across the lineages. These studies indicate that, after duplication of an ancestral BRC1-like gene, BRC1b genes continued to evolve under a strong purifying selection that was consistent with the conserved function of SlBRC1b in shoot branching control. In contrast, the coding sequences of Solanum BRC1a genes have evolved at a higher evolution rate. Branch-site tests indicate that this difference does not reflect relaxation but rather positive selective pressure for adaptation.</p>1 aMartín-Trillo, Mar1 aGrandío, Eduardo, González1 aSerra, François1 aMarcel, Fabien1 aRodríguez-Buey, María, Luisa1 aSchmitz, Gregor1 aTheres, Klaus1 aBendahmane, Abdelhafid1 aDopazo, Hernán1 aCubas, Pilar  uhttps://www.clinbioinfosspa.es/content/role-tomato-branched1-genes-control-shoot-branching