02545nas a2200253   4500008004100000022001400041245011200055210006900167260001300236300001100249490000700260520175500267100001902022700002002041700001702061700002002078700001702098700002102115700002302136700002202159700001802181700002102199856007102220       2015                        eng d  a1872-914200aTherapeutic targets for olive pollen allergy defined by gene markers modulated by Ole e 1-derived peptides.0 aTherapeutic targets for olive pollen allergy defined by gene mar  c2015 Apr  a252-610 v643 aTwo regions of Ole e 1, the major olive-pollen allergen, have been characterized as T-cell epitopes, one as immunodominant region (aa91-130) and the other, as mainly recognized by non-allergic subjects (aa10-31). This report tries to characterize the specific relevance of these epitopes in the allergic response to olive pollen by analyzing the secreted cytokines and the gene expression profiles induced after specific stimulation of peripheral blood mononuclear cells (PBMCs). PBMCs from olive pollen-allergic and non-allergic control subjects were stimulated with olive-pollen extract and Ole e 1 dodecapeptides containing relevant T-cell epitopes. Levels of cytokines were measured in cellular supernatants and gene expression was determined by microarrays, on the RNAs extracted from PBMCs. One hundred eighty-nine differential genes (fold change >2 or <-2, P<0.05) were validated by qRT-PCR in a large population. It was not possible to define a pattern of response according the overall cytokine results but interesting differences were observed, mainly in the regulatory cytokines. Principal component (PCA) gene-expression analysis defined clusters that correlated with the experimental conditions in the group of allergic subjects. Gene expression and functional analyses revealed differential genes and pathways among the experimental conditions. A set of 51 genes (many essential to T-cell tolerance and homeostasis) correlated with the response to aa10-31 of Ole e 1. In conclusion, two peptides derived from Ole e 1 could regulate the immune response in allergic patients, by gene-expression modification of several regulation-related genes. These results open new research ways to the regulation of allergy by Oleaceae family members.1 aCalzada, David1 aAguerri, Miriam1 aBaos, Selene1 aMontaner, David1 aMata, Manuel1 aDopazo, Joaquín1 aQuiralte, Joaquín1 aFlorido, Fernando1 aLahoz, Carlos1 aCárdaba, Blanca  uhttp://www.sciencedirect.com/science/article/pii/S0161589014003356