03013nas a2200433 4500008004100000022001400041245016000055210006900215260001300284300001200297490000700309520160000316653001601916653003801932653001501970653001701985653001902002653002702021653001502048653002602063653002602089653001002115100002402125700002402149700001902173700002002192700002202212700002102234700002202255700002902277700002002306700001802326700002002344700002402364700001902388700002102407700001902428856013202447 2017 eng d a1573-502800aIntegration of transcriptomic and metabolic data reveals hub transcription factors involved in drought stress response in sunflower (Helianthus annuus L.).0 aIntegration of transcriptomic and metabolic data reveals hub tra c2017 Jul a549-5640 v943 a
By integration of transcriptional and metabolic profiles we identified pathways and hubs transcription factors regulated during drought conditions in sunflower, useful for applications in molecular and/or biotechnological breeding. Drought is one of the most important environmental stresses that effects crop productivity in many agricultural regions. Sunflower is tolerant to drought conditions but the mechanisms involved in this tolerance remain unclear at the molecular level. The aim of this study was to characterize and integrate transcriptional and metabolic pathways related to drought stress in sunflower plants, by using a system biology approach. Our results showed a delay in plant senescence with an increase in the expression level of photosynthesis related genes as well as higher levels of sugars, osmoprotectant amino acids and ionic nutrients under drought conditions. In addition, we identified transcription factors that were upregulated during drought conditions and that may act as hubs in the transcriptional network. Many of these transcription factors belong to families implicated in the drought response in model species. The integration of transcriptomic and metabolomic data in this study, together with physiological measurements, has improved our understanding of the biological responses during droughts and contributes to elucidate the molecular mechanisms involved under this environmental condition. These findings will provide useful biotechnological tools to improve stress tolerance while maintaining crop yield under restricted water availability.
10aChlorophyll10aGene Expression Regulation, Plant10aHelianthus10aPlant Leaves10aPlant Proteins10aProtein Array Analysis10aRNA, Plant10aStress, Physiological10aTranscription Factors10aWater1 aMoschen, Sebastián1 aDi Rienzo, Julio, A1 aHiggins, Janet1 aTohge, Takayuki1 aWatanabe, Mutsumi1 aGonzalez, Sergio1 aRivarola, Máximo1 aGarcia-Garcia, Francisco1 aDopazo, Joaquin1 aHopp, Esteban1 aHoefgen, Rainer1 aFernie, Alisdair, R1 aPaniego, Norma1 aFernandez, Paula1 aHeinz, Ruth, A uhttps://www.clinbioinfosspa.es/content/integration-transcriptomic-and-metabolic-data-reveals-hub-transcription-factors-involved