01816nas a2200145   4500008004100000245010300041210006900144260001600213300000800229490000700237520125000244100001801494700002401512856013401536       2010                        eng d00aQuantifying the relationship between sequence and three-dimensional structure conservation in RNA.0 aQuantifying the relationship between sequence and threedimension  c2010 Jun 15  a3220 v113 a<p>ABSTRACT: BACKGROUND: In recent years, the number of available RNA structures has rapidly grown reflecting the increased interest on RNA biology. Similarly to the studies carried out two decades ago for proteins, which gave the fundamental grounds for developing comparative protein structure prediction methods, we are now able to quantify the relationship between sequence and structure conservation in RNA. RESULTS: Here we introduce an all-against-all sequence- and three-dimensional (3D) structure-based comparison of a representative set of RNA structures, which have allowed us to quantitatively confirm that: (i) there is a measurable relationship between sequence and structure conservation that weakens for alignments resulting in below 60% sequence identity, (ii) evolution tends to conserve more RNA structure than sequence, and (iii) there is a twilight zone for RNA homology detection. DISCUSSION: The computational analysis here presented quantitatively describes the relationship between sequence and structure for RNA molecules and defines a twilight zone region for detecting RNA homology. Our work could represent the theoretical basis and limitations for future developments in comparative RNA 3D structure prediction.</p>1 aCapriotti, E.1 aMarti-Renom, M., A.  uhttps://www.clinbioinfosspa.es/content/quantifying-relationship-between-sequence-and-three-dimensional-structure-conservation-rna