@article {806,
	title = {Primary Ciliary Dyskinesia and Retinitis Pigmentosa: Novel  Variant and Possible Modifier Gene.},
	journal = {Cells},
	volume = {13},
	year = {2024},
	month = {2024 Mar 16},
	abstract = {<p>We report a novel  missense variant co-segregated with a familial X-linked retinitis pigmentosa (XLRP) case. The brothers were hemizygous for this variant, but only the proband presented with primary ciliary dyskinesia (PCD). Thus, we aimed to elucidate the role of the  variant and other modifier genes in the phenotypic variability observed in the family and its impact on motile cilia. The pathogenicity of the variant on the RPGR protein was evaluated by in vitro studies transiently transfecting the mutated  gene, and immunofluorescence analysis on nasal brushing samples. Whole-exome sequencing was conducted to identify potential modifier variants. In vitro studies showed that the mutated RPGR protein could not localise to the cilium and impaired cilium formation. Accordingly, RPGR was abnormally distributed in the siblings{\textquoteright} nasal brushing samples. In addition, a missense variant in  was identified. The concurrent  variant influenced ciliary mislocalisation of the protein. We provide a comprehensive characterisation of motile cilia in this XLRP family, with only the proband presenting PCD symptoms. The variant{\textquoteright}s pathogenicity was confirmed, although it alone does not explain the respiratory symptoms. Finally, the  gene may be a potential modifier for respiratory symptoms in patients with  mutations.</p>},
	keywords = {Ciliary Motility Disorders, Eye Proteins, Genes, Modifier, Humans, Male, mutation, Retinitis pigmentosa},
	issn = {2073-4409},
	doi = {10.3390/cells13060524},
	author = {Baz-Red{\'o}n, Noelia and S{\'a}nchez-Bellver, Laura and Fern{\'a}ndez-Cancio, M{\'o}nica and Rovira-Amigo, Sandra and Burgoyne, Thomas and Ranjit, Rai and Aquino, Virginia and Toro-Barrios, Noem{\'\i} and Carmona, Rosario and Polverino, Eva and Cols, Maria and Moreno-Gald{\'o}, Antonio and Camats-Tarruella, N{\'u}ria and Marfany, Gemma}
}
