@article {493,
	title = {The Activation of the Sox2 RR2 Pluripotency Transcriptional Reporter in Human Breast Cancer Cell Lines is Dynamic and Labels Cells with Higher Tumorigenic Potential.},
	journal = {Front Oncol},
	volume = {4},
	year = {2014},
	month = {2014},
	pages = {308},
	abstract = {<p>The striking similarity displayed at the mechanistic level between tumorigenesis and the generation of induced pluripotent stem cells and the fact that genes and pathways relevant for embryonic development are reactivated during tumor progression highlights the link between pluripotency and cancer. Based on these observations, we tested whether it is possible to use a pluripotency-associated transcriptional reporter, whose activation is driven by the SRR2 enhancer from the Sox2 gene promoter (named S4+ reporter), to isolate cancer stem cells (CSCs) from breast cancer cell lines. The S4+ pluripotency transcriptional reporter allows the isolation of cells with enhanced tumorigenic potential and its activation was switched on and off in the cell lines studied, reflecting a plastic cellular process. Microarray analysis comparing the populations in which the reporter construct is active versus inactive showed that positive cells expressed higher mRNA levels of cytokines (IL-8, IL-6, TNF) and genes (such as ATF3, SNAI2, and KLF6) previously related with the CSC phenotype in breast cancer. </p>},
	issn = {2234-943X},
	doi = {10.3389/fonc.2014.00308},
	author = {Iglesias, Juan Manuel and Leis, Olatz and P{\'e}rez Ruiz, Est{\'\i}baliz and Gumuzio Barrie, Juan and Garcia-Garcia, Francisco and Aduriz, Ariane and Beloqui, Izaskun and Hernandez-Garcia, Susana and Lopez-Mato, Maria Paz and Dopazo, Joaquin and Pandiella, Atanasio and Menendez, Javier A and Martin, Angel Garcia}
}
