%0 Journal Article %J Omics : a journal of integrative biology %D 2013 %T Assessing Differential Expression Measurements by Highly Parallel Pyrosequencing and DNA Microarrays: A Comparative Study. %A Ariño, Joaquín %A Casamayor, Antonio %A Pérez, Julián Perez %A Pedrola, Laia %A Alvarez-Tejado, Miguel %A Marbà, Martina %A Santoyo, Javier %A Joaquín Dopazo %X

Abstract To explore the feasibility of pyrosequencing for quantitative differential gene expression analysis we have performed a comparative study of the results of the sequencing experiments to those obtained by a conventional DNA microarray platform. A conclusion from our analysis is that, over a threshold of 35 normalized reads per gene, the measurements of gene expression display a good correlation with the references. The observed concordance between pyrosequencing and DNA microarray platforms beyond the threshold was of 0.8, measured as a Pearson’s correlation coefficient. In differential gene expression the initial aim is the quantification the differences among transcripts when comparing experimental conditions. Thus, even in a scenario of low coverage the concordance in the measurements is quite acceptable. On the other hand, the comparatively longer read size obtained by pyrosequencing allows detecting unconventional splicing forms.

%B Omics : a journal of integrative biology %8 2011 Sep 15 %G eng %U http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3545353/ %R 10.1089/omi.2011.0065