%0 Journal Article %J DNA Res %D 2016 %T Highly sensitive and ultrafast read mapping for RNA-seq analysis. %A Medina, I %A Tárraga, J %A Martínez, H %A Barrachina, S %A Castillo, M I %A Paschall, J %A Salavert-Torres, J %A Blanquer-Espert, I %A Hernández-García, V %A Quintana-Ortí, E S %A Dopazo, J %K Genomics %K High-Throughput Nucleotide Sequencing %K Humans %K Sensitivity and Specificity %K Sequence Analysis, RNA %K Transcriptome %X

As sequencing technologies progress, the amount of data produced grows exponentially, shifting the bottleneck of discovery towards the data analysis phase. In particular, currently available mapping solutions for RNA-seq leave room for improvement in terms of sensitivity and performance, hindering an efficient analysis of transcriptomes by massive sequencing. Here, we present an innovative approach that combines re-engineering, optimization and parallelization. This solution results in a significant increase of mapping sensitivity over a wide range of read lengths and substantial shorter runtimes when compared with current RNA-seq mapping methods available.

%B DNA Res %V 23 %P 93-100 %8 2016 Apr %G eng %N 2 %1 https://www.ncbi.nlm.nih.gov/pubmed/26740642?dopt=Abstract %R 10.1093/dnares/dsv039