01698nas a2200337 4500008004100000022001400041245007000055210006800125260001300193300001100206490000700217520067800224653001300902653004200915653001100957653003200968653002701000653001801027100001401045700001601059700001701075700001801092700001901110700001601129700002301145700002301168700002601191700002501217700001401242856010401256 2016 eng d a1756-166300aHighly sensitive and ultrafast read mapping for RNA-seq analysis.0 aHighly sensitive and ultrafast read mapping for RNAseq analysis c2016 Apr a93-1000 v233 a
As sequencing technologies progress, the amount of data produced grows exponentially, shifting the bottleneck of discovery towards the data analysis phase. In particular, currently available mapping solutions for RNA-seq leave room for improvement in terms of sensitivity and performance, hindering an efficient analysis of transcriptomes by massive sequencing. Here, we present an innovative approach that combines re-engineering, optimization and parallelization. This solution results in a significant increase of mapping sensitivity over a wide range of read lengths and substantial shorter runtimes when compared with current RNA-seq mapping methods available.
10aGenomics10aHigh-Throughput Nucleotide Sequencing10aHumans10aSensitivity and Specificity10aSequence Analysis, RNA10aTranscriptome1 aMedina, I1 aTárraga, J1 aMartínez, H1 aBarrachina, S1 aCastillo, M, I1 aPaschall, J1 aSalavert-Torres, J1 aBlanquer-Espert, I1 aHernández-García, V1 aQuintana-Ortí, E, S1 aDopazo, J uhttps://www.clinbioinfosspa.es/content/highly-sensitive-and-ultrafast-read-mapping-rna-seq-analysis