| Title | Highly sensitive and ultrafast read mapping for RNA-seq analysis. |
| Publication Type | Journal Article |
| Year of Publication | 2016 |
| Authors | Medina, I, Tárraga, J, Martínez, H, Barrachina, S, Castillo, MI, Paschall, J, Salavert-Torres, J, Blanquer-Espert, I, Hernández-García, V, Quintana-Ortí, ES, Dopazo, J |
| Journal | DNA Res |
| Volume | 23 |
| Issue | 2 |
| Pagination | 93-100 |
| Date Published | 2016 Apr |
| ISSN | 1756-1663 |
| Keywords | Genomics; High-Throughput Nucleotide Sequencing; Humans; Sensitivity and Specificity; Sequence Analysis, RNA; Transcriptome |
| Abstract | As sequencing technologies progress, the amount of data produced grows exponentially, shifting the bottleneck of discovery towards the data analysis phase. In particular, currently available mapping solutions for RNA-seq leave room for improvement in terms of sensitivity and performance, hindering an efficient analysis of transcriptomes by massive sequencing. Here, we present an innovative approach that combines re-engineering, optimization and parallelization. This solution results in a significant increase of mapping sensitivity over a wide range of read lengths and substantial shorter runtimes when compared with current RNA-seq mapping methods available. |
| DOI | 10.1093/dnares/dsv039 |
| Alternate Journal | DNA Res |
| PubMed ID | 26740642 |
| PubMed Central ID | PMC4833417 |
